• Western blot analysis of extracts of various cell lines, using IRF9 antibody (STJ24232) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IRF9 antibody (113-255) (STJ24232)

SKU:
STJ24232

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IRF9 (113-255) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IRF9
Gene ID: 10379
Uniprot ID: IRF9_HUMAN
Immunogen Region: 113-255
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 113-255 of human IRF9 (NP_006075.3).
Immunogen Sequence: QLLPPGIVSGQPGTQKVPSK RQHSSVSSERKEEEDAMQNC TLSPSVLQDSLNNEEEGASG GAVHSDIGSSSSSSSPEPQE VTDTTEAPFQGDQRSLEFLL PPEPDYSLLLTFIYNGRVVG EAQVQSLDCRLVAEPSGSES SME
Post Translational Modifications (Microbial infection) Ubiquitinated by Herpes simplex virus 2 E3 ubiquitin ligase ICP22.
Function Transcription factor that plays an essential role in anti-viral immunity. It mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Protein Name Interferon Regulatory Factor 9
Irf-9
Ifn-Alpha-Responsive Transcription Factor Subunit
Isgf3 P48 Subunit
Interferon-Stimulated Gene Factor 3 Gamma
Isgf-3 Gamma
Transcriptional Regulator Isgf3 Subunit Gamma
Database Links Reactome: R-HSA-877300
Reactome: R-HSA-909733
Cellular Localisation Cytoplasm
Nucleus
Translocated Into The Nucleus Upon Activation By Ifn-Alpha/Beta
Alternative Antibody Names Anti-Interferon Regulatory Factor 9 antibody
Anti-Irf-9 antibody
Anti-Ifn-Alpha-Responsive Transcription Factor Subunit antibody
Anti-Isgf3 P48 Subunit antibody
Anti-Interferon-Stimulated Gene Factor 3 Gamma antibody
Anti-Isgf-3 Gamma antibody
Anti-Transcriptional Regulator Isgf3 Subunit Gamma antibody
Anti-IRF9 antibody
Anti-ISGF3G antibody

Information sourced from Uniprot.org

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