| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-INPP5B (1-260) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
INPP5B |
| Gene ID: |
3633 |
| Uniprot ID: |
I5P2_HUMAN |
| Immunogen Region: |
1-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human INPP5B (NP_005531.2). |
| Immunogen Sequence: |
MDQSVAIQETLAEGEYCVIA VQGVLCEGDSRQSRLLGLVR YRLEHGGQEHALFLYTHRRM AITGDDVSLDQIVPVSRDFT LEEVSPDGELYILGSDVTVQ LDTAELSLVFQLPFGSQTRM FLHEVARACPGFDSATRDPE FLWLSRYRCAELELEMPTPR GCNSALVTWPGYATIGGGGS NFDGLRPNGKGVPMDQSSRG QDKPESLQPRQNKSKSEITD MVRSSTITVSDKAHILSMQ |
| Tissue Specificity | Platelets. |
| Post Translational Modifications | Isoprenylation at Cys-990 may be required for localization at the membrane. May be proteolytically cleaved after Lys-320 as inferred from N-terminal protein sequence of the 75 kda form. |
| Function | Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events. |
| Protein Name | Type Ii Inositol 1 -4 -5-Trisphosphate 5-Phosphatase75 Kda Inositol Polyphosphate-5-PhosphatasePhosphoinositide 5-Phosphatase5ptase |
| Database Links | Reactome: R-HSA-1855183Reactome: R-HSA-1855204 |
| Cellular Localisation | CytoplasmCytosolEndoplasmic Reticulum-Golgi Intermediate CompartmentEarly Endosome MembraneMembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmic VesiclePhagosome MembraneGolgi Apparatus |
| Alternative Antibody Names | Anti-Type Ii Inositol 1 -4 -5-Trisphosphate 5-Phosphatase antibodyAnti-75 Kda Inositol Polyphosphate-5-Phosphatase antibodyAnti-Phosphoinositide 5-Phosphatase antibodyAnti-5ptase antibodyAnti-INPP5B antibodyAnti-OCRL2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
