• Western blot analysis of extracts of HeLa cells, using IL18 antibody (STJ119148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of C6 cells using IL18 antibody (STJ119148) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using IL18 antibody (STJ119148) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using IL18 antibody (STJ119148) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IL18 antibody (31-130) (STJ119148)

SKU:
STJ119148

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL18 (31-130) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL18
Gene ID: 3606
Uniprot ID: IL18_HUMAN
Immunogen Region: 31-130
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 31-130 of human IL18 (NP_001553.1).
Immunogen Sequence: ENLESDYFGKLESKLSVIRN LNDQVLFIDQGNRPLFEDMT DSDCRDNAPRTIFIISMYKD SQPRGMAVTISVKCEKISTL SCENKIISFKEMNPPDNIKD
Post Translational Modifications The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form.
Function Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Protein Name Interleukin-18
Il-18
Iboctadekin
Interferon Gamma-Inducing Factor
Ifn-Gamma-Inducing Factor
Interleukin-1 Gamma
Il-1 Gamma
Database Links Reactome: R-HSA-448706
Reactome: R-HSA-5620971
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-9012546 Q14116-2
Reactome: R-HSA-9660826
Cellular Localisation Cytoplasm
Secreted
The Precursor Is Cytosolic
In Response To Inflammasome-Activating Signals
Cleaved And Secreted
Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore
In Contrast
The Precursor Form Is Not Released
Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
Alternative Antibody Names Anti-Interleukin-18 antibody
Anti-Il-18 antibody
Anti-Iboctadekin antibody
Anti-Interferon Gamma-Inducing Factor antibody
Anti-Ifn-Gamma-Inducing Factor antibody
Anti-Interleukin-1 Gamma antibody
Anti-Il-1 Gamma antibody
Anti-IL18 antibody
Anti-IGIF antibody
Anti-IL1F4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance