| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-IL13 (25-146) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IL13 |
| Gene ID: |
3596 |
| Uniprot ID: |
IL13_HUMAN |
| Immunogen Region: |
25-146 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 25-146 of human IL13 (NP_002179.2). |
| Immunogen Sequence: |
LTCLGGFASPGPVPPSTALR ELIEELVNITQNQKAPLCNG SMVWSINLTAGMYCAALESL INVSGCSAIEKTQRMLSGFC PHKVSAGQFSSLHVRDTKIE VAQFVKDLLLHLKKLFREGQ FN |
| Function | Cytokine that plays important roles in allergic inflammation and immune response to parasite infection. Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells. Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1. Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B. Functions also on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils. Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6. Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13. |
| Protein Name | Interleukin-13Il-13 |
| Database Links | Reactome: R-HSA-6785807Reactome: R-HSA-9012546 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Interleukin-13 antibodyAnti-Il-13 antibodyAnti-IL13 antibodyAnti-NC30 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and IL13 Rabbit polyclonal antibody knockdown (KD) MCF7 cells, using [KD Validated] IL13 Rabbit polyclonal antibody (STJ11104717) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/177361/369056/STJ11104717_1__78862.1713134541.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IL13 Rabbit polyclonal antibody knockdown (KD) MCF7 cells, using [KD Validated] IL13 Rabbit polyclonal antibody (STJ11104717) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
