• Western blot analysis of extracts of various cell lines, using (STJ11103657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Confocal imaging of K-562 cells using IL11RA Rabbit monoclonal antibody (STJ11103657, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of extracts of Rat brain, using (STJ11103657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Confocal imaging of RAW 264. 7 cells using IL11RA Rabbit monoclonal antibody (STJ11103657, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded human liver using IL11RA rabbit monoclonal antibody (STJ11103657) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IL11RA in paraffin-embedded rat spleen tissue using IL11RA Rabbit monoclonal antibody (STJ11103657) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human lung cancer using IL11RA rabbit monoclonal antibody (STJ11103657) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IL11RA in paraffin-embedded mouse brain tissue using IL11RA Rabbit monoclonal antibody (STJ11103657) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse spinal cord using IL11RA rabbit monoclonal antibody (STJ11103657) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IL11RA in paraffin-embedded human liver cancer tissue using IL11RA Rabbit monoclonal antibody (STJ11103657) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded rat kidney using IL11RA rabbit monoclonal antibody (STJ11103657) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IL11RA in paraffin-embedded human colon tissue using IL11RA Rabbit monoclonal antibody (STJ11103657) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded rat spleen using IL11RA rabbit monoclonal antibody (STJ11103657) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat brain, using (STJ11103657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of TF-1 cells using IL11RA antibody (STJ11103657) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using (STJ11103657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of RAW264. 7 cells using IL11RA antibody (STJ11103657) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IL11RA antibody (323-422) [S7MR] (STJ11103657)

SKU:
STJ11103657

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IL11RA (323-422) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL11RA
Gene ID: 3590
Uniprot ID: I11RA_HUMAN
Immunogen Region: 323-422
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 323-422 of human IL11RA (Q14626).
Immunogen Sequence: EIPAWGQLHTQPEVEPQVDS PAPPRPSLQPHPRLLDHRDS VEQVAVLASLGILSFLGLVA GALALGLWLRLRRGGKDGSP KPGFLASVIPVDRRPGAPNL
Tissue Specificity Expressed in a number of cell lines, including the myelogenous leukemia cell line K-562, the megakaryocytic leukemia cell line M-07e, the erythroleukemia cell line TF-1, and the osteosarcoma cell lines, MG-63 and SaOS-2. Also expressed in normal and malignant prostate epithelial cell lines. Expression levels are increased in prostate carcinoma.
Post Translational Modifications A short soluble form is also released from the membrane by proteolysis. The sIL11RA is formed either by limited proteolysis of membrane-bound receptors, a process referred to as ectodomain shedding, or directly secreted from the cells after alternative mRNA splicing. mIL11RA is cleaved by the proteases ADAM10, ELANE and PRTN3.
Function Receptor for interleukin-11 (IL11). The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and/or differentiation of skeletogenic progenitor or other mesenchymal cells (Probable). Essential for the normal development of craniofacial bones and teeth. Restricts suture fusion and tooth number. Soluble interleukin-11 receptor subunit alpha: Soluble form of IL11 receptor (sIL11RA) that acts as an agonist of IL11 activity. The IL11:sIL11RA complex binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL11RA in a process called IL11 trans-signaling. Isoform HCR2: Soluble form of IL11 receptor (sIL11RA) that acts as an agonist of IL11 activity. The IL11:sIL11RA complex binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL11RA in a process called IL11 trans-signaling.
Protein Name Interleukin-11 Receptor Subunit Alpha
Il-11 Receptor Subunit Alpha
Il-11r Subunit Alpha
Il-11r-Alpha
Il-11ra Cleaved Into - Soluble Interleukin-11 Receptor Subunit Alpha
Sil-11r
Sil-11ra
Sil11ra
Database Links Reactome: R-HSA-6788467
Cellular Localisation Interleukin-11 Receptor Subunit Alpha: Membrane
Single-Pass Type I Membrane Protein
Soluble Interleukin-11 Receptor Subunit Alpha: Secreted
Isoform Hcr2: Secreted
Alternative Antibody Names Anti-Interleukin-11 Receptor Subunit Alpha antibody
Anti-Il-11 Receptor Subunit Alpha antibody
Anti-Il-11r Subunit Alpha antibody
Anti-Il-11r-Alpha antibody
Anti-Il-11ra Cleaved Into - Soluble Interleukin-11 Receptor Subunit Alpha antibody
Anti-Sil-11r antibody
Anti-Sil-11ra antibody
Anti-Sil11ra antibody
Anti-IL11RA antibody

Information sourced from Uniprot.org

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