| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-IFT74 (1-372) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IFT74 |
| Gene ID: |
80173 |
| Uniprot ID: |
IFT74_HUMAN |
| Immunogen Region: |
1-372 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-372 of human IFT74 (NP_001092694.1). |
| Immunogen Sequence: |
MASNHKSSAARPVSRGGVGL TGRPPSGIRPLSGNIRVATA MPPGTARPGSRGCPIGTGGV LSSQIKVAHRPVTQQGLTGM KTGTKGPQRQILDKSYYLGL LRSKISELTTEVNKLQKGIE MYNQENSVYLSYEKRAETLA VEIKELQGQLADYNMLVDKL NTNTEMEEVMNDYNMLKAQN DRETQSLDVIFTERQAKEKQ IRSVEEEIEQEKQATDDIIK NMSFENQVKYLEMKTTNEK |
| Tissue Specificity | Highly expressed in adult and fetal kidney and expressed at lower level in adult heart, placenta, lung, liver and pancreas, and in fetal heart, lung and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus and spleen. Detected in sperm (at protein level). |
| Function | Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis. Essential for flagellogenesis during spermatogenesis. |
| Protein Name | Intraflagellar Transport Protein 74 HomologCapillary Morphogenesis Gene 1 ProteinCmg-1Coiled-Coil Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-5620924 |
| Cellular Localisation | Cell ProjectionCiliumCytoplasmic VesicleFlagellumSecretory VesicleAcrosomeLocalizes Along Primary Cilia At Interphase And Around The Basal Body/Centriole At Interphase And MitosisIn Male Germ CellsStrongly Expressed In The Vesicles Of Spermatocytes And Round Spermatids And Also In The Acrosome And Centrosome Regions Of Elongating Spermatids And In Developing Sperm Tails |
| Alternative Antibody Names | Anti-Intraflagellar Transport Protein 74 Homolog antibodyAnti-Capillary Morphogenesis Gene 1 Protein antibodyAnti-Cmg-1 antibodyAnti-Coiled-Coil Domain-Containing Protein 2 antibodyAnti-IFT74 antibodyAnti-CCDC2 antibodyAnti-CMG1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and hnRNP A1 knockout (KO) 293T cells, using [KO Validated] hnRNP A1 Rabbit polyclonal antibody (STJ11100067) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89137/357586/STJ11100067_1__30225.1713121721.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and hnRNP A1 knockout (KO) 293T cells, using [KO Validated] hnRNP A1 Rabbit polyclonal antibody (STJ11100067) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
