• Western blot analysis of extracts of various cell lines, using IDH3G antibody (STJ115696) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IDH3G antibody (40-170) (STJ115696)

SKU:
STJ115696

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IDH3G (40-170) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IDH3G
Gene ID: 3421
Uniprot ID: IDH3G_HUMAN
Immunogen Region: 40-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-170 of human IDH3G (NP_004126.1).
Immunogen Sequence: FSEQTIPPSAKYGGRHTVTM IPGDGIGPELMLHVKSVFRH ACVPVDFEEVHVSSNADEED IRNAIMAIRRNRVALKGNIE TNHNLPPSHKSRNNILRTSL DLYANVIHCKSLPGVVTRHK DIDILIVRENT
Function Regulatory subunit which plays a role in the allosteric regulation of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
Protein Name Isocitrate Dehydrogenase Nad Subunit Gamma - Mitochondrial
Isocitric Dehydrogenase Subunit Gamma
Nad(+-Specific Icdh Subunit Gamma
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Isocitrate Dehydrogenase Nad Subunit Gamma - Mitochondrial antibody
Anti-Isocitric Dehydrogenase Subunit Gamma antibody
Anti-Nad(+-Specific Icdh Subunit Gamma antibody
Anti-IDH3G antibody

Information sourced from Uniprot.org

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