• Immunofluorescence analysis of NIH-3T3 cells using IDH2 Rabbit monoclonal antibody (STJ11101307) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using IDH2 Rabbit monoclonal antibody (STJ11101307) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using IDH2 Rabbit monoclonal antibody (STJ11101307) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using IDH2 Rabbit monoclonal antibody (STJ11101307) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3s.

Anti-IDH2 antibody (353-452) [S7MR] (STJ11101307)

SKU:
STJ11101307

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IDH2 (353-452) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IDH2
Gene ID: 3418
Uniprot ID: IDHP_HUMAN
Immunogen Region: 353-452
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 353-452 of human IDH2 (P48735).
Immunogen Sequence: RHYREHQKGRPTSTNPIASI FAWTRGLEHRGKLDGNQDLI RFAQMLEKVCVETVESGAMT KDLAGCIHGLSNVKLNEHFL NTTDFLDTIKSNLDRALGRQ
Post Translational Modifications Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
Function Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Protein Name Isocitrate Dehydrogenase Nadp - Mitochondrial
Idh
Icd-M
Idp
Nadp(+-Specific Icdh
Oxalosuccinate Decarboxylase
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Isocitrate Dehydrogenase Nadp - Mitochondrial antibody
Anti-Idh antibody
Anti-Icd-M antibody
Anti-Idp antibody
Anti-Nadp(+-Specific Icdh antibody
Anti-Oxalosuccinate Decarboxylase antibody
Anti-IDH2 antibody

Information sourced from Uniprot.org

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