• Immunofluorescence analysis of paraffin-embedded rat brain using Huntingtin Rabbit monoclonal antibody (STJ11101602) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded mouse brain using Huntingtin Rabbit monoclonal antibody (STJ11101602) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Huntingtin in paraffin-embedded human brain tissue using Huntingtin Rabbit monoclonal antibody (STJ11101602) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Huntingtin in paraffin-embedded mouse brain tissue using Huntingtin Rabbit monoclonal antibody (STJ11101602) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Huntingtin in paraffin-embedded rat brain tissue using Huntingtin Rabbit monoclonal antibody (STJ11101602) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Huntingtin in paraffin-embedded human brain tissue using Huntingtin Rabbit monoclonal antibody (STJ11101602) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Huntingtin in paraffin-embedded mouse spinal cord using Huntingtin Rabbit monoclonal antibody (STJ11101602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Huntingtin Rabbit monoclonal antibody (STJ11101602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of various lysates using Huntingtin Rabbit monoclonal antibody (STJ11101602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HTT antibody (1-100) [S2MR] (STJ11101602)

SKU:
STJ11101602

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Huntingtin (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTT
Gene ID: 3064
Uniprot ID: HD_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Huntingtin (P42858).
Immunogen Sequence: MATLEKLMKAFESLKSFQQQ QQQQQQQQQQQQQQQQQQPP PPPPPPPPPQLPQPPPQAQP LLPQPQPPPPPPPPPPGPAV AEEPLHRPKKELSATKKDRV
Tissue Specificity Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
Post Translational Modifications Huntingtin: Cleaved by caspases downstream of the polyglutamine stretch. The resulting N-terminal fragments are cytotoxic and provokes apoptosis. Huntingtin: Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation. Huntingtin: Phosphorylation at Ser-1179 and Ser-1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Huntingtin, myristoylated N-terminal fragment: Myristoylated at Gly-551, following proteolytic cleavage at Asp-550.
Function Huntingtin: May play a role in microtubule-mediated transport or vesicle function. Huntingtin, myristoylated N-terminal fragment: Promotes the formation of autophagic vesicles.
Protein Name Huntingtin
Huntington Disease Protein
Hd Protein Cleaved Into - Huntingtin - Myristoylated N-Terminal Fragment
Database Links Reactome: R-HSA-9022692
Cellular Localisation Huntingtin: Cytoplasm
Nucleus
Early Endosome
The Mutant Huntingtin Protein Colocalizes With Akap8l In The Nuclear Matrix Of Huntington Disease Neurons
Shuttles Between Cytoplasm And Nucleus In A Ran Gtpase-Independent Manner
Recruits Onto Early Endosomes In A Rab5- And Hap40-Dependent Fashion
Huntingtin
Myristoylated N-Terminal Fragment: Cytoplasmic Vesicle
Autophagosome
Alternative Antibody Names Anti-Huntingtin antibody
Anti-Huntington Disease Protein antibody
Anti-Hd Protein Cleaved Into - Huntingtin - Myristoylated N-Terminal Fragment antibody
Anti-HTT antibody
Anti-HD antibody
Anti-IT15 antibody

Information sourced from Uniprot.org

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