• Immunofluorescence analysis of C6 cells using HTATSF1 Rabbit polyclonal antibody (STJ116753) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HTATSF1 in paraffin-embedded mouse testis using HTATSF1 Rabbit polyclonal antibody (STJ116753) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HTATSF1 in paraffin-embedded human gastric cancer using HTATSF1 Rabbit polyclonal antibody (STJ116753) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HTATSF1 in paraffin-embedded human lung cancer using HTATSF1 Rabbit polyclonal antibody (STJ116753) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HTATSF1 Rabbit polyclonal antibody (STJ116753) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HTATSF1 antibody (1-180) (STJ116753)

SKU:
STJ116753

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HTATSF1 (1-180) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTATSF1
Gene ID: 27336
Uniprot ID: HTSF1_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HTATSF1 (NP_055315.2).
Immunogen Sequence: MSGTNLDGNDEFDEQLRMQE LYGDGKDGDTQTDAGGEPDS LGQQPTDTPYEWDLDKKAWF PKITEDFIATYQANYGFSND GASSSTANVEDVHARTAEEP PQEKAPEPTDARKKGEKRKA ESGWFHVEEDRNTNVYVSGL PPDITVDEFIQLMSKFGIIM RDPQTEEFKVKLYKDNQGNL
Tissue Specificity Widely expressed.
Function Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus.
Protein Name Hiv Tat-Specific Factor 1
Tat-Sf1
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hiv Tat-Specific Factor 1 antibody
Anti-Tat-Sf1 antibody
Anti-HTATSF1 antibody

Information sourced from Uniprot.org

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