• Western blot analysis of extracts from normal (control) and HSP105/HSP105/HSPH1 knockout (KO) 293T cells, using HSP105/HSP105/HSPH1 antibody (STJ11100790) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSPH1 antibody (659-858) (STJ11100790)

SKU:
STJ11100790

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSPH1 (659-858) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPH1
Gene ID: 10808
Uniprot ID: HS105_HUMAN
Immunogen Region: 659-858
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 659-858 of human HSP105/HSP105/HSPH1 (NP_006635.2).
Immunogen Sequence: EQDHQNFLRLLTETEDWLYE EGEDQAKQAYVDKLEELMKI GTPVKVRFQEAEERPKMFEE LGQRLQHYAKIAADFRNKDE KYNHIDESEMKKVEKSVNEV MEWMNNVMNAQAKKSLDQDP VVRAQEIKTKIKELNNTCEP VVTQPKPKIESPKLERTPNG PNIDKKEEDLEDKNNFGAEP PHQNGECYPNEKNSVNMDLD
Tissue Specificity Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
Post Translational Modifications Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
Function Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release. Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
Protein Name Heat Shock Protein 105 Kda
Antigen Ny-Co-25
Heat Shock 110 Kda Protein
Database Links Reactome: R-HSA-3000484
Reactome: R-HSA-3371453
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Heat Shock Protein 105 Kda antibody
Anti-Antigen Ny-Co-25 antibody
Anti-Heat Shock 110 Kda Protein antibody
Anti-HSPH1 antibody
Anti-HSP105 antibody
Anti-HSP110 antibody
Anti-KIAA0201 antibody

Information sourced from Uniprot.org

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