• Western blot analysis of extracts of mouse liver, using HNF4A antibody (STJ115933) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-HNF4A antibody (155-254) (STJ115933)

SKU:
STJ115933

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HNF4A (155-254) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNF4A
Gene ID: 3172
Uniprot ID: HNF4A_HUMAN
Immunogen Region: 155-254
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 155-254 of human HNF4A (NP_000448.3).
Immunogen Sequence: LLQAEVLSRQITSPVSGING DIRAKKIASIADVCESMKEQ LLVLVEWAKYIPAFCELPLD DQVALLRAHAGEHLLLGATK RSMVFKDVLLLGNDYIVPRH
Post Translational Modifications Phosphorylated on tyrosine residue(s).phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA. Acetylation at Lys-458 lowers transcriptional activation by about two-fold.
Function Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.
Protein Name Hepatocyte Nuclear Factor 4-Alpha
Hnf-4-Alpha
Nuclear Receptor Subfamily 2 Group A Member 1
Transcription Factor 14
Tcf-14
Transcription Factor Hnf-4
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-383280
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hepatocyte Nuclear Factor 4-Alpha antibody
Anti-Hnf-4-Alpha antibody
Anti-Nuclear Receptor Subfamily 2 Group A Member 1 antibody
Anti-Transcription Factor 14 antibody
Anti-Tcf-14 antibody
Anti-Transcription Factor Hnf-4 antibody
Anti-HNF4A antibody
Anti-HNF4 antibody
Anti-NR2A1 antibody
Anti-TCF14 antibody

Information sourced from Uniprot.org

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