• Western blot analysis of various lysates using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit polyclonal antibody (STJ113462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HMOX1 antibody (1-288) (STJ113462)

SKU:
STJ113462

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HO-1 (1-288) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMOX1
Gene ID: 3162
Uniprot ID: HMOX1_HUMAN
Immunogen Region: 1-288
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human Heme Oxygenase 1 (HO-1/HMOX1) (NP_002124.1).
Immunogen Sequence: MERPQPDSMPQDLSEALKEA TKEVHTQAENAEFMRNFQKG QVTRDGFKLVMASLYHIYVA LEEEIERNKESPVFAPVYFP EELHRKAALEQDLAFWYGPR WQEVIPYTPAMQRYVKRLHE VGRTEPELLVAHAYTRYLGD LSGGQVLKKIAQKALDLPSS GEGLAFFTFPNIASATKFKQ LYRSRMNSLEMTPAVRQRVI EEAKTAFLLNIQLFEELQEL LTHDTKDQSPSRAPGLRQR
Tissue Specificity Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).
Post Translational Modifications A soluble form arises by proteolytic removal of the membrane anchor.
Function Heme oxygenase 1: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis. Heme oxygenase 1: (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. Heme oxygenase 1 soluble form: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
Protein Name Heme Oxygenase 1
Ho-1 Cleaved Into - Heme Oxygenase 1 Soluble Form
Database Links Reactome: R-HSA-189483
Reactome: R-HSA-6785807
Reactome: R-HSA-844456
Reactome: R-HSA-917937
Reactome: R-HSA-9609523
Reactome: R-HSA-9660826
Reactome: R-HSA-9707564
Reactome: R-HSA-9707587
Reactome: R-HSA-9707616
Reactome: R-HSA-9818027
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iv Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Heme Oxygenase 1 antibody
Anti-Ho-1 Cleaved Into - Heme Oxygenase 1 Soluble Form antibody
Anti-HMOX1 antibody
Anti-HO antibody
Anti-HO1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance