• Western blot analysis of various lysates, using HLF Rabbit polyclonal antibody (STJ24033) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HLF antibody (1-295) (STJ24033)

SKU:
STJ24033

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HLF (1-295) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HLF
Gene ID: 3131
Uniprot ID: HLF_HUMAN
Immunogen Region: 1-295
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human HLF (NP_002117.1).
Immunogen Sequence: MEKMSRPLPLNPTFIPPPYG VLRSLLENPLKLPLHHEDAF SKDKDKEKKLDDESNSPTVP QSAFLGPTLWDKTLPYDGDT FQLEYMDLEEFLSENGIPPS PSQHDHSPHPPGLQPASSAA PSVMDLSSRASAPLHPGIPS PNCMQSPIRPGQLLPANRNT PSPIDPDTIQVPVGYEPDPA DLALSSIPGQEMFDPRKRKF SEEELKPQPMIKKARKVFIP DDLKDDKYWARRRKNNMAA
Tissue Specificity Highly expressed in liver.lower levels in lung and kidney.
Protein Name Hepatic Leukemia Factor
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hepatic Leukemia Factor antibody
Anti-HLF antibody

Information sourced from Uniprot.org

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