• Western blot analysis of extracts of various cell lines, using HLA-DRB5 antibody (STJ114602) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HLA-DRB5 antibody (30-227) (STJ114602)

SKU:
STJ114602

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HLA-DRB5 (30-227) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HLA-DRB5
Gene ID: 3127
Uniprot ID: DRB5_HUMAN
Immunogen Region: 30-227
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-227 of human HLA-DRB5 (NP_002116.2).
Immunogen Sequence: GDTRPRFLQQDKYECHFFNG TERVRFLHRDIYNQEEDLRF DSDVGEYRAVTELGRPDAEY WNSQKDFLEDRRAAVDTYCR HNYGVGESFTVQRRVEPKVT VYPARTQTLQHHNLLVCSVN GFYPGSIEVRWFRNSQEEKA GVVSTGLIQNGDWTFQTLVM LETVPRSGEVYTCQVEHPSV TSPLTVEWRAQSESAQSK
Post Translational Modifications Ubiquitinated by MARCH1 and MARCH8 at Lys-254 leading to down-regulation of MHC class II.
Function Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Protein Name Hla Class Ii Histocompatibility Antigen - Dr Beta 5 Chain
Dr Beta-5
Dr2-Beta-2
Dw2
Mhc Class Ii Antigen Drb5
Database Links Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-2132295
Reactome: R-HSA-389948
Reactome: R-HSA-877300
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum Membrane
Golgi Apparatus
Trans-Golgi Network Membrane
Endosome Membrane
Lysosome Membrane
Late Endosome Membrane
The Mhc Class Ii Complex Transits Through A Number Of Intracellular Compartments In The Endocytic Pathway Until It Reaches The Cell Membrane For Antigen Presentation
Alternative Antibody Names Anti-Hla Class Ii Histocompatibility Antigen - Dr Beta 5 Chain antibody
Anti-Dr Beta-5 antibody
Anti-Dr2-Beta-2 antibody
Anti-Dw2 antibody
Anti-Mhc Class Ii Antigen Drb5 antibody
Anti-HLA-DRB5 antibody

Information sourced from Uniprot.org

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