• Western blot analysis of extracts of various cell lines, using HK1 antibody (STJ11103454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.
  • Western blot analysis of extracts of various cell lines, using HK1 antibody (STJ11103454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of U-2 OS using HK1 antibody (STJ11103454) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A549 using HK1 antibody (STJ11103454) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HK1 antibody (21-219) [S4MR] (STJ11103454)

SKU:
STJ11103454

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HK1 (21-219) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HK1
Gene ID: 3098
Uniprot ID: HXK1_HUMAN
Immunogen Region: 21-219
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-219 of human HK1 (NP_000179.2).
Immunogen Sequence: KIDKYLYAMRLSDETLIDIM TRFRKEMKNGLSRDFNPTAT VKMLPTFVRSIPDGSEKGDF IALDLGGSSFRILRVQVNHE KNQNVHMESEVYDTPENIVH GSGSQLFDHVAECLGDFMEK RKIKDKKLPVGFTFSFPCQQ SKIDEAILITWTKRFKASGV EGADVVKLLNKAIKKRGDYD ANIVAVVNDTVGTMMTCGY
Tissue Specificity Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific. Isoform 4: Testis-specific.
Function Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome.
Protein Name Hexokinase-1
Brain Form Hexokinase
Hexokinase Type I
Hk I
Hexokinase-A
Database Links Reactome: R-HSA-5619056
Reactome: R-HSA-70171
Cellular Localisation Mitochondrion Outer Membrane
Peripheral Membrane Protein
Cytoplasm
Cytosol
The Mitochondrial-Binding Peptide (Mbp) Region Promotes Association With The Mitochondrial Outer Membrane (Probable)
Dissociates From The Mitochondrial Outer Membrane Following Inhibition By N-Acetyl-D-Glucosamine
Leading To Relocation To The Cytosol
Alternative Antibody Names Anti-Hexokinase-1 antibody
Anti-Brain Form Hexokinase antibody
Anti-Hexokinase Type I antibody
Anti-Hk I antibody
Anti-Hexokinase-A antibody
Anti-HK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance