• Immunofluorescence analysis of U-2 OS cells using Histone H1. 0 antibody (STJ28998) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Histone H1. 0 antibody (STJ28998) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Histone H1 antibody (1-194) (STJ28998)

SKU:
STJ28998

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Histone H1.0 (1-194) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H1-0
Gene ID: 3005
Uniprot ID: H10_HUMAN
Immunogen Region: 1-194
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human H1F0 (NP_005309.1).
Immunogen Sequence: MTENSTSAPAAKPKRAKASK KSTDHPKYSDMIVAAIQAEK NRAGSSRQSIQKYIKSHYKV GENADSQIKLSIKRLVTTGV LKQTKGVGASGSFRLAKSDE PKKSVAFKKTKKEIKKVATP KKASKPKKAASKAPTKKPKA TPVKKAKKKLAATPKKAKKP KTVKAKPVKASKPKKAKPVK PKAKSSAKRAGKKK
Post Translational Modifications Phosphorylated on Ser-17 in RNA edited version. ADP-ribosylated on Ser-104 in response to DNA damage.
Function Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Protein Name Histone H1.0
Histone H1'
Histone H1(0 Cleaved Into - Histone H1.0 - N-Terminally Processed
Database Links Reactome: R-HSA-140342
Reactome: R-HSA-2559584
Cellular Localisation Nucleus
Chromosome
The Rna Edited Version Has Been Localized To Nuclear Speckles
During Mitosis
It Appears In The Vicinity Of Condensed Chromosomes
Alternative Antibody Names Anti-Histone H1.0 antibody
Anti-Histone H1' antibody
Anti-Histone H1(0 Cleaved Into - Histone H1.0 - N-Terminally Processed antibody
Anti-H1-0 antibody
Anti-H1F0 antibody
Anti-H1FV antibody

Information sourced from Uniprot.org

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