• Western blot analysis of extracts of various cell lines, using HIC2 antibody (STJ116525) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HIC2 antibody (120-420) (STJ116525)

SKU:
STJ116525

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HIC2 (120-420) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HIC2
Gene ID: 23119
Uniprot ID: HIC2_HUMAN
Immunogen Region: 120-420
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-420 of human HIC2 (NP_055909.2).
Immunogen Sequence: LTAASYLQLPELAALCRRKL KRAGKPFGSGRAGSTGMGRP PRSQRLSTASVIQARYQGLV DGRKGAHAPQELPQAKGSDD ELFLGGSNQDSVQGLGRAVC PAGGEAGLGGCSSSTNGSSG GCEQELGLDLSKKSPPLPPA TPGPHLTPDDAAQLSDSQHG SPPAASAPPVANSASYSELG GTPDEPMDLEGAEDNHLSLL EAPGGQPRKSLRHSTRKKEW GKKEPVAGSPFERREAGPK
Tissue Specificity Highest levels in cerebellum.
Function Transcriptional repressor.
Protein Name Hypermethylated In Cancer 2 Protein
Hic-2
Hic1-Related Gene On Chromosome 22 Protein
Hic-3
Zinc Finger And Btb Domain-Containing Protein 30
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hypermethylated In Cancer 2 Protein antibody
Anti-Hic-2 antibody
Anti-Hic1-Related Gene On Chromosome 22 Protein antibody
Anti-Hic-3 antibody
Anti-Zinc Finger And Btb Domain-Containing Protein 30 antibody
Anti-HIC2 antibody
Anti-HRG22 antibody
Anti-KIAA1020 antibody
Anti-ZBTB30 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance