• Western blot analysis of various lysates, using HHLA2 rabbit polyclonal antibody (STJ115227) at 1:3500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using HHLA2 antibody (STJ115227) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung using HHLA2 antibody (STJ115227) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using HHLA2 antibody (STJ115227) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HHLA2 antibody (30-200) (STJ115227)

SKU:
STJ115227

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HHLA2 (30-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HHLA2
Gene ID: 11148
Uniprot ID: HHLA2_HUMAN
Immunogen Region: 30-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human HHLA2 (NP_009003.1).
Immunogen Sequence: IYVPMNEQIVIGRLDEDIIL PSSFERGSEVVIHWKYQDSY KVHSYYKGSDHLESQDPRYA NRTSLFYNEIQNGNASLFFR RVSLLDEGIYTCYVGTAIQV ITNKVVLKVGVFLTPVMKYE KRNTNSFLICSVLSVYPRPI ITWKMDNTPISENNMEETGS LDSFSINSPLN
Tissue Specificity Expressed at high levels in colon, kidney, testis, lung and pancreas, and at lower levels in small intestine, liver and skeletal muscle. In immune cells, highly expressed in B-cells, dendritic cells and macrophages. Not detected in T-cells.
Function Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
Protein Name Herv-H Ltr-Associating Protein 2
Human Endogenous Retrovirus-H Long Terminal Repeat-Associating Protein 2
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Herv-H Ltr-Associating Protein 2 antibody
Anti-Human Endogenous Retrovirus-H Long Terminal Repeat-Associating Protein 2 antibody
Anti-HHLA2 antibody

Information sourced from Uniprot.org

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