• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g Lsh/HELLS antibody (STJ28394). Western blot was performed from the immunoprecipitate using Lsh/HELLS antibody (STJ28394) at a dilution of 1:1000.
  • Western blot analysis of lysates from wild type (WT) and Lsh/HELLS knockout (KO) HeLa cells, using [KO Validated] Lsh/HELLS Rabbit polyclonal antibody (STJ28394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates using [KO Validated] Lsh/HELLS Rabbit polyclonal antibody (STJ28394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HELLS antibody (719-838) (STJ28394)

SKU:
STJ28394

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HELLS (719-838) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HELLS
Gene ID: 3070
Uniprot ID: HELLS_HUMAN
Immunogen Region: 719-838
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human Lsh/HELLS (NP_060533.2).
Immunogen Sequence: VYRLVTANTIDQKIVERAAA KRKLEKLIIHKNHFKGGQSG LNLSKNFLDPKELMELLKSR DYEREIKGSREKVISDKDLE LLLDRSDLIDQMNASGPIKE KMGIFKILENSEDSSPECLF
Tissue Specificity Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias.
Function Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis.
Protein Name Lymphoid-Specific Helicase
Proliferation-Associated Snf2-Like Protein
Swi/Snf2-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 6
Cellular Localisation Nucleus
Closely Associated With Pericentric Heterochromatin
Alternative Antibody Names Anti-Lymphoid-Specific Helicase antibody
Anti-Proliferation-Associated Snf2-Like Protein antibody
Anti-Swi/Snf2-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 6 antibody
Anti-HELLS antibody
Anti-PASG antibody
Anti-SMARCA6 antibody
Anti-Nbla10143 antibody

Information sourced from Uniprot.org

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