• Western blot analysis of extracts from wild type (WT) and HDAC10 knockdown (KD) 293T (KD) cells, using HDAC10 Rabbit polyclonal antibody (STJ11105309) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of MCF7, using HDAC10 Rabbit polyclonal antibody (STJ11105309) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HDAC10 antibody (STJ11105309)

SKU:
STJ11105309

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Polyamine Deacetylase Hdac10 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC10
Gene ID: 83933
Uniprot ID: HDA10_HUMAN
Immunogen: A synthesized peptide derived from human HDAC10.
Immunogen Sequence: DQPCLCPAPSVRTAVALTTP DITLVLPPDVIQQEASALRE ETEAWARPHESLAREEALTA LGKLLYLLDGMLDGQVNSGI AATPASAAAATLDVAVRRGL SHGAQRLLCVALGQLDRPPD LAHDGRSLWLNIRGKEAAAL SMFHVSTPLPVMTGGFLSCI LGLVLPLAYGFQPDLVLVAL GPGHGLQGPHAALLAAMLRG LAGGRVLALLEENSTPQLAG ILARVLNGEAPPSLGPSSV
Tissue Specificity Widely expressed with high levels in liver and kidney.
Function Polyamine deacetylase (PDAC), which acts preferentially on N(8)-acetylspermidine, and also on acetylcadaverine and acetylputrescine. Exhibits attenuated catalytic activity toward N(1),N(8)-diacetylspermidine and very low activity, if any, toward N(1)-acetylspermidine. Histone deacetylase activity has been observed in vitro. Has also been shown to be involved in MSH2 deacetylation. The physiological relevance of protein/histone deacetylase activity is unclear and could be very weak. May play a role in the promotion of late stages of autophagy, possibly autophagosome-lysosome fusion and/or lysosomal exocytosis in neuroblastoma cells. May play a role in homologous recombination. May promote DNA mismatch repair.
Protein Name Polyamine Deacetylase Hdac10
Histone Deacetylase 10
Hd10
Database Links Reactome: R-HSA-2122947
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-3214815
Reactome: R-HSA-350054
Cellular Localisation Cytoplasm
Nucleus
Excluded From Nucleoli
Alternative Antibody Names Anti-Polyamine Deacetylase Hdac10 antibody
Anti-Histone Deacetylase 10 antibody
Anti-Hd10 antibody
Anti-HDAC10 antibody

Information sourced from Uniprot.org

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