• Immunohistochemistry analysis of paraffin-embedded mouse kidney using HCLS1 antibody (STJ23925) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using HCLS1 antibody (STJ23925) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using HCLS1 antibody (STJ23925) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using HCLS1 antibody (STJ23925) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-HCLS1 antibody (1-280) (STJ23925)

SKU:
STJ23925

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HCLS1 (1-280) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HCLS1
Gene ID: 3059
Uniprot ID: HCLS1_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HCLS1 (NP_005326.2).
Immunogen Sequence: MWKSVVGHDVSVSVETQGDD WDTDPDFVNDISEKEQRWGA KTIEGSGRTEHINIHQLRNK VSEEHDVLRKKEMESGPKAS HGYGGRFGVERDRMDKSAVG HEYVAEVEKHSSQTDAAKGF GGKYGVERDRADKSAVGFDY KGEVEKHTSQKDYSRGFGGR YGVEKDKWDKAALGYDYKGE TEKHESQRDYAKGFGGQYGI QKDRVDKSAVGFNEMEAPTT AYKKTTPIEAASSGTRGLK
Tissue Specificity Expressed only in tissues and cells of hematopoietic origin.
Post Translational Modifications Phosphorylated by FES. Phosphorylated by LYN, FYN and FGR after cross-linking of surface IgM on B-cells. Phosphorylation by LYN, FYN and FGR requires prior phosphorylation by SYK or FES.
Function Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
Protein Name Hematopoietic Lineage Cell-Specific Protein
Hematopoietic Cell-Specific Lyn Substrate 1
Lckbp1
P75
Cellular Localisation Membrane
Peripheral Membrane Protein
Cytoplasm
Mitochondrion
Alternative Antibody Names Anti-Hematopoietic Lineage Cell-Specific Protein antibody
Anti-Hematopoietic Cell-Specific Lyn Substrate 1 antibody
Anti-Lckbp1 antibody
Anti-P75 antibody
Anti-HCLS1 antibody
Anti-HS1 antibody

Information sourced from Uniprot.org

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