• Western blot analysis of extracts of various cell lines, using HBS1L antibody (STJ112077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-HBS1L antibody (1-260) (STJ112077)

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STJ112077

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HBS1L (1-260) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HBS1L
Gene ID: 10767
Uniprot ID: HBS1L_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human HBS1L (NP_006611.1).
Immunogen Sequence: MARHRNVRGYNYDEDFEDDD LYGQSVEDDYCISPSTAAQF IYSRRDKPSVEPVEEYDYED LKESSNSVSNHQLSGFDQAR LYSCLDHMREVLGDAVPDEI LIEAVLKNKFDVQKALSGVL EQDRVQSLKDKNEATVSTGK IAKGKPVDSQTSRSESEIVP KVAKMTVSGKKQTMGFEVPG VSSEENGHSFHTPQKGPPIE DAIASSDVLETASKSANPPH TIQASEEQSSTPAPVKKSG
Tissue Specificity Detected in heart, brain, placenta, liver, muscle, kidney and pancreas.
Function GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.
Protein Name Hbs1-Like Protein
Erfs
Database Links Reactome: R-HSA-429958
Cellular Localisation Cytoplasm
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Hbs1-Like Protein antibody
Anti-Erfs antibody
Anti-HBS1L antibody
Anti-HBS1 antibody
Anti-KIAA1038 antibody

Information sourced from Uniprot.org

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