| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HBS1L (1-260) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HBS1L |
| Gene ID: |
10767 |
| Uniprot ID: |
HBS1L_HUMAN |
| Immunogen Region: |
1-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human HBS1L (NP_006611.1). |
| Immunogen Sequence: |
MARHRNVRGYNYDEDFEDDD LYGQSVEDDYCISPSTAAQF IYSRRDKPSVEPVEEYDYED LKESSNSVSNHQLSGFDQAR LYSCLDHMREVLGDAVPDEI LIEAVLKNKFDVQKALSGVL EQDRVQSLKDKNEATVSTGK IAKGKPVDSQTSRSESEIVP KVAKMTVSGKKQTMGFEVPG VSSEENGHSFHTPQKGPPIE DAIASSDVLETASKSANPPH TIQASEEQSSTPAPVKKSG |
| Tissue Specificity | Detected in heart, brain, placenta, liver, muscle, kidney and pancreas. |
| Function | GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway. |
| Protein Name | Hbs1-Like ProteinErfs |
| Database Links | Reactome: R-HSA-429958 |
| Cellular Localisation | CytoplasmIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Hbs1-Like Protein antibodyAnti-Erfs antibodyAnti-HBS1L antibodyAnti-HBS1 antibodyAnti-KIAA1038 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")