• Immunohistochemistry analysis of paraffin-embedded human gastric cancer using HAPLN1 antibody (STJ114303) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using HAPLN1 antibody (STJ114303) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using HAPLN1 antibody (STJ114303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HAPLN1 antibody (165-354) (STJ114303)

SKU:
STJ114303

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HAPLN1 (165-354) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HAPLN1
Gene ID: 1404
Uniprot ID: HPLN1_HUMAN
Immunogen Region: 165-354
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 165-354 of human HAPLN1 (NP_001875.1).
Immunogen Sequence: PRLGRYNLNFHEAQQACLDQ DAVIASFDQLYDAWRGGLDW CNAGWLSDGSVQYPITKPRE PCGGQNTVPGVRNYGFWDKD KSRYDVFCFTSNFNGRFYYL IHPTKLTYDEAVQACLNDGA QIAKVGQIFAAWKILGYDRC DAGWLADGSVRYPISRPRRR CSPTEAAVRFVGFPDKKHKL YGVYCFRAYN
Tissue Specificity Widely expressed. Weakly expressed in the brain.
Function Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
Protein Name Hyaluronan And Proteoglycan Link Protein 1
Cartilage-Linking Protein 1
Cartilage-Link Protein
Proteoglycan Link Protein
Database Links Reactome: R-HSA-3000178
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Hyaluronan And Proteoglycan Link Protein 1 antibody
Anti-Cartilage-Linking Protein 1 antibody
Anti-Cartilage-Link Protein antibody
Anti-Proteoglycan Link Protein antibody
Anti-HAPLN1 antibody
Anti-CRTL1 antibody

Information sourced from Uniprot.org

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