• Confocal imaging of paraffin-embedded Mouse brain tissue using GluR2/GRIA2 Rabbit monoclonal antibody (STJ11101312, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat brain tissue using GluR2/GRIA2 Rabbit monoclonal antibody (STJ11101312, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of GluR2/GRIA2 in paraffin-embedded mouse brain using GluR2/GRIA2 Rabbit monoclonal antibody (STJ11101312) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GluR2/GRIA2 in paraffin-embedded rat brain using GluR2/GRIA2 Rabbit monoclonal antibody (STJ11101312) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GluR2/GRIA2 Rabbit monoclonal antibody (STJ11101312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GRIA2 antibody (150-250) [S2MR] (STJ11101312)

SKU:
STJ11101312

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GluR2 (150-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRIA2
Gene ID: 2891
Uniprot ID: GRIA2_HUMAN
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human GluR2/GRIA2 (P42262).
Immunogen Sequence: YLYDSDRGLSTLQAVLDSAA EKKWQVTAINVGNINNDKKD EMYRSLFQDLELKKERRVIL DCERDKVNDIVDQVITIGKH VKGYHYIIANLGFTDGDLLK I
Post Translational Modifications Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. Phosphorylation at Tyr-876 is required forc interaction with IQSEC1 and ARF6 activation. Ubiquitinated by RNF167, leading to its degradation.
Function Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system. It plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting.
Protein Name Glutamate Receptor 2
Glur-2
Ampa-Selective Glutamate Receptor 2
Glur-B
Glur-K2
Glutamate Receptor Ionotropic - Ampa 2
Glua2
Database Links Reactome: R-HSA-399710
Reactome: R-HSA-416993
Reactome: R-HSA-438066
Reactome: R-HSA-9022699
Reactome: R-HSA-9620244
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Postsynaptic Cell Membrane
Postsynaptic Density Membrane
Interaction With Cacng2
Cnih2 And Cnih3 Promotes Cell Surface Expression
Displays A Somatodendritic Localization And Is Excluded From Axons In Neurons
Alternative Antibody Names Anti-Glutamate Receptor 2 antibody
Anti-Glur-2 antibody
Anti-Ampa-Selective Glutamate Receptor 2 antibody
Anti-Glur-B antibody
Anti-Glur-K2 antibody
Anti-Glutamate Receptor Ionotropic - Ampa 2 antibody
Anti-Glua2 antibody
Anti-GRIA2 antibody
Anti-GLUR2 antibody

Information sourced from Uniprot.org

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