• Western blot analysis of extracts of various cell lines, using GPSM1 antibody (STJ113580) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using GPSM1 Rabbit polyclonal antibody (STJ113580) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GPSM1 antibody (158-457) (STJ113580)

SKU:
STJ113580

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPSM1
Gene ID: 26086
Uniprot ID: GPSM1_HUMAN
Immunogen Region: 158-457
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 158-457 of human GPSM1 (NP_056412.5).
Immunogen Sequence: AKGKQLSWNAANATQDPGHL PPDVRETLCKASEFYERNLS LVKELGDRAAQGRAYGNLGN THYLLGNFTEATTFHKERLA IAKEFGDKAAERRAYSNLGN AHVFLGRFDVAAEYYKKTLQ LSRQLRDQAVEAQACYSLGN TYTLLQDYERAAEYHLRHLL IAQELADRVGEGRACWSLGN AYVSMGRPAQALTFAKKHLQ ISQEIGDRHGELTARMNVAQ LQLVLGRLTSPAASEKPDL
Tissue Specificity Expressed in intestinal cells.
Post Translational Modifications Phosphorylation regulates interaction with G(i/o) alpha.
Function Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.
Protein Name G-Protein-Signaling Modulator 1
Activator Of G-Protein Signaling 3
Database Links Reactome: R-HSA-418594
Cellular Localisation Cytoplasm
Cytosol
Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Golgi Apparatus Membrane
Cell Membrane
Alternative Antibody Names Anti-G-Protein-Signaling Modulator 1 antibody
Anti-Activator Of G-Protein Signaling 3 antibody
Anti-GPSM1 antibody
Anti-AGS3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance