• Western blot analysis of extracts of mouse heart, using GPAA1 antibody (STJ115792) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GPAA1 antibody (90-310) (STJ115792)

SKU:
STJ115792

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPAA1 (90-310) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPAA1
Gene ID: 8733
Uniprot ID: GPAA1_HUMAN
Immunogen Region: 90-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 90-310 of human GPAA1 (NP_003792.1).
Immunogen Sequence: AWLERTMRSVGLEVYTQSFS RKLPFPDETHERYMVSGTNV YGILRAPRAASTESLVLTVP CGSDSTNSQAVGLLLALAAH FRGQIYWAKDIVFLVTEHDL LGTEAWLEAYHDVNVTGMQS SPLQGRAGAIQAAVALELSS DVVTSLDVAVEGLNGQLPNL DLLNLFQTFCQKGGLLCTLQ GKLQPEDWTSLDGPLQGLQT LLLMVLRQASGRPHGSHGLF L
Tissue Specificity Ubiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues.
Function Component of the GPI transamidase complex, necessary for transfer of GPI to proteins. Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate.
Protein Name Glycosylphosphatidylinositol Anchor Attachment 1 Protein
Gpi Anchor Attachment Protein 1
Gaa1 Protein Homolog
Hgaa1
Database Links Reactome: R-HSA-162791
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Glycosylphosphatidylinositol Anchor Attachment 1 Protein antibody
Anti-Gpi Anchor Attachment Protein 1 antibody
Anti-Gaa1 Protein Homolog antibody
Anti-Hgaa1 antibody
Anti-GPAA1 antibody
Anti-GAA1 antibody

Information sourced from Uniprot.org

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