• Western blot analysis of extracts of various cell lines, using Glucosylceramidase beta (Glucosylceramidase beta (GBA) ) Rabbit polyclonal antibody (STJ11102788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Glucosylceramidase beta antibody (40-250) (STJ11102788)

SKU:
STJ11102788

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Glucosylceramidase beta (GBA) (40-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GBA1
Gene ID: 2629
Uniprot ID: GBA1_HUMAN
Immunogen Region: 40-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human Glucosylceramidase beta (Glucosylceramidase beta (GBA) ) (NP_000148.2).
Immunogen Sequence: ARPCIPKSFGYSSVVCVCNA TYCDSFDPPTFPALGTFSRY ESTRSGRRMELSMGPIQANH TGTGLLLTLQPEQKFQKVKG FGGAMTDAAALNILALSPPA QNLLLKSYFSEEGIGYNIIR VPMASCDFSIRTYTYADTPD DFQLHNFSLPEEDTKLKIPL IHRALQLAQRPVSLLASPWT SPTWLKTNGAVNGKGSLKGQ PGDIYHQTWAR
Function Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramides/GlcCers (such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine) into free ceramides (such as N-acylsphing-4-enine) and glucose. Plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through the production of ceramides, participates in the PKC-activated salvage pathway of ceramide formation. Catalyzes the glucosylation of cholesterol, through a transglucosylation reaction where glucose is transferred from GlcCer to cholesterol. GlcCer containing mono-unsaturated fatty acids (such as beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4-enine) are preferred as glucose donors for cholesterol glucosylation when compared with GlcCer containing same chain length of saturated fatty acids (such as beta-D-glucosyl-N-octadecanoyl-sphing-4-enine). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl 3-beta-D-glucoside to ceramide (Probable). Can also hydrolyze cholesteryl 3-beta-D-glucoside producing glucose and cholesterol. Catalyzes the hydrolysis of galactosylceramides/GalCers (such as beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine), as well as the transfer of galactose between GalCers and cholesterol in vitro, but with lower activity than with GlcCers. Contrary to GlcCer and GalCer, xylosylceramide/XylCer (such as beta-D-xyosyl-(1<->1')-N-acylsphing-4-enine) is not a good substrate for hydrolysis, however it is a good xylose donor for transxylosylation activity to form cholesteryl 3-beta-D-xyloside.
Protein Name Lysosomal Acid Glucosylceramidase
Lysosomal Acid Gcase
Acid Beta-Glucosidase
Alglucerase
Beta-Glucocerebrosidase
Beta-Gc
Beta-Glucosylceramidase 1
Cholesterol Glucosyltransferase
Sgtase
Cholesteryl-Beta-Glucosidase
D-Glucosyl-N-Acylsphingosine Glucohydrolase
Glucosylceramidase Beta 1
Imiglucerase
Lysosomal Cholesterol Glycosyltransferase
Lysosomal Galactosylceramidase
Lysosomal Glycosylceramidase
Database Links Reactome: R-HSA-1660662
Reactome: R-HSA-390471
Cellular Localisation Lysosome Membrane
Peripheral Membrane Protein
Lumenal Side
Interaction With Saposin-C Promotes Membrane Association
Targeting To Lysosomes Occurs Through An Alternative Mpr-Independent Mechanism Via Scarb2
Alternative Antibody Names Anti-Lysosomal Acid Glucosylceramidase antibody
Anti-Lysosomal Acid Gcase antibody
Anti-Acid Beta-Glucosidase antibody
Anti-Alglucerase antibody
Anti-Beta-Glucocerebrosidase antibody
Anti-Beta-Gc antibody
Anti-Beta-Glucosylceramidase 1 antibody
Anti-Cholesterol Glucosyltransferase antibody
Anti-Sgtase antibody
Anti-Cholesteryl-Beta-Glucosidase antibody
Anti-D-Glucosyl-N-Acylsphingosine Glucohydrolase antibody
Anti-Glucosylceramidase Beta 1 antibody
Anti-Imiglucerase antibody
Anti-Lysosomal Cholesterol Glycosyltransferase antibody
Anti-Lysosomal Galactosylceramidase antibody
Anti-Lysosomal Glycosylceramidase antibody
Anti-GBA1 antibody
Anti-GBA antibody
Anti-GC antibody
Anti-GLUC antibody

Information sourced from Uniprot.org

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