| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Zinc finger protein GLI1 (460-490 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:10000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GLI1 |
| Gene ID: | 2735 |
| Uniprot ID: | GLI1_HUMAN |
| Immunogen Region: | 460-490 aa |
| Specificity: | This antibody detects endogenous levels of GLI-1 |
| Immunogen: | Synthesized peptide derived from the human GLI-1 at the amino acid range 460-490 |
| Post Translational Modifications | Phosphorylated in vitro by ULK3. Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1. Ubiquitinated by the CRL2(FEM1B) complex, suppressing GLI1 transcriptional activator activity. |
| Function | Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling. Isoform 2: Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration. |
| Protein Name | Zinc Finger Protein Gli1 Glioma-Associated Oncogene Oncogene Gli |
| Database Links | Reactome: R-HSA-5610780 Reactome: R-HSA-5610787 Reactome: R-HSA-5632684 Reactome: R-HSA-5635851 |
| Cellular Localisation | Cytoplasm Nucleus Tethered In The Cytoplasm By Binding To Sufu Activation And Translocation To The Nucleus Is Promoted By Interaction With Stk36 Phosphorylation By Ulk3 May Promote Nuclear Localization Translocation To The Nucleus Is Promoted By Interaction With Zic1 Isoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Zinc Finger Protein Gli1 antibody Anti-Glioma-Associated Oncogene antibody Anti-Oncogene Gli antibody Anti-GLI1 antibody Anti-GLI antibody |
Information sourced from Uniprot.org