• Western blot analysis of extracts of various cell lines, using GJB4 Antibody (STJ111930) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GJB4 antibody (100-200) (STJ111930)

SKU:
STJ111930

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GJB4 (100-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GJB4
Gene ID: 127534
Uniprot ID: CXB4_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human GJB4 (NP_694944.1).
Immunogen Sequence: ERERKHHLKHGPNAPSLYDN LSKKRGGLWWTYLLSLIFKA AVDAGFLYIFHRLYKDYDMP RVVACSVEPCPHTVDCYISR PTEKKVFTYFMVTTAAICIL L
Function Structural component of gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.
Protein Name Gap Junction Beta-4 Protein
Connexin-30.3
Cx30.3
Database Links Reactome: R-HSA-190861
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Gap Junction
Colocalizes With Gjb2 At Gap Junction Plaques In The Cochlea
Alternative Antibody Names Anti-Gap Junction Beta-4 Protein antibody
Anti-Connexin-30.3 antibody
Anti-Cx30.3 antibody
Anti-GJB4 antibody

Information sourced from Uniprot.org

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