• Immunofluorescence analysis of HepG2 cells using GFRA1 Rabbit polyclonal antibody (STJ27326) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of GFRA1 in paraffin-embedded rat brain using GFRA1 Rabbit polyclonal antibody (STJ27326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GFRA1 in paraffin-embedded mouse spinal cord using GFRA1 Rabbit polyclonal antibody (STJ27326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GFRA1 in paraffin-embedded human liver using GFRA1 Rabbit polyclonal antibody (STJ27326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GFRA1 Rabbit polyclonal antibody (STJ27326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GFRA1 antibody (345-420) (STJ27326)

SKU:
STJ27326

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GFRA1
Gene ID: 2674
Uniprot ID: GFRA1_HUMAN
Immunogen Region: 345-420
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 345-420 of human GFRA1 (NP_005255.1).
Immunogen Sequence: FGNGSDVTVWQPAFPVQTTT ATTTTALRVKNKPLGPAGSE NEIPTHVLPPCANLQAQKLK SNVSGNTHLCISNGNY
Function Coreceptor for GDNF, a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. GDNF-binding leads to autophosphorylation and activation of the RET receptor.
Protein Name Gdnf Family Receptor Alpha-1
Gdnf Receptor Alpha-1
Gdnfr-Alpha-1
Gfr-Alpha-1
Ret Ligand 1
Tgf-Beta-Related Neurotrophic Factor Receptor 1
Database Links Reactome: R-HSA-419037
Reactome: R-HSA-5673001
Reactome: R-HSA-8853659
Reactome: R-HSA-9830674
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Golgi Apparatus
Trans-Golgi Network
Endosome
Multivesicular Body
Localizes Mainly To The Plasma Membrane
In The Presence Of Sorl1
Shifts To Vesicular Structures
Including Trans-Golgi Network
Endosomes And Multivesicular Bodies
Alternative Antibody Names Anti-Gdnf Family Receptor Alpha-1 antibody
Anti-Gdnf Receptor Alpha-1 antibody
Anti-Gdnfr-Alpha-1 antibody
Anti-Gfr-Alpha-1 antibody
Anti-Ret Ligand 1 antibody
Anti-Tgf-Beta-Related Neurotrophic Factor Receptor 1 antibody
Anti-GFRA1 antibody
Anti-GDNFRA antibody
Anti-RETL1 antibody
Anti-TRNR1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance