• Western blot analysis using GATA-1 monoclonal antibody against K562 (1) cell lysate.

Anti-GATA1 antibody [4G1] (STJ98097)

SKU:
STJ98097

Current Stock:
Host: Mouse
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Erythroid transcription factor is suitable for use in Western Blot and ELISA research applications.
Clonality: Monoclonal
Clone ID: 4G1
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATA1
Gene ID: 2623
Uniprot ID: GATA1_HUMAN
Specificity: GATA-1 Monoclonal Antibody detects endogenous levels of GATA-1 protein.
Immunogen: Purified recombinant fragment of human GATA-1 expressed in E. Coli.
Post Translational Modifications Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137. Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity. Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo. Acetylated on Lys-233, Lys-245 Lys-246 by EP300.
Function Transcriptional activator or repressor which serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-ATGATAAG-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.
Protein Name Erythroid Transcription Factor
Eryf1
Gata-Binding Factor 1
Gata-1
Gf-1
Nf-E1 Dna-Binding Protein
Database Links Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Erythroid Transcription Factor antibody
Anti-Eryf1 antibody
Anti-Gata-Binding Factor 1 antibody
Anti-Gata-1 antibody
Anti-Gf-1 antibody
Anti-Nf-E1 Dna-Binding Protein antibody
Anti-GATA1 antibody
Anti-ERYF1 antibody
Anti-GF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance