Western blot analysis of Jurkat, using GARS antibody (STJ11101248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GARS antibody (640-739) [S8MR] (STJ11101248)

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STJ11101248
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GARS1
Gene ID: 2617
Uniprot ID: GARS_HUMAN
Immunogen Region: 640-739
Specificity: A synthetic peptide corresponding to a sequence within amino acids 640-739 of human GARS (P41250).
Immunogen Sequence: RHGVSHKVDDSSGSIGRRYA RTDEIGVAFGVTIDFDTVNK TPHTATLRDRDSMRQIRAEI SELPSIVQDLANGNITWADV EARYPLFEGQETGKKETIEE
Tissue Specificity Widely expressed, including in brain and spinal cord. Isoform 2: Expressed in brain, spinal cord, muscle, heart and spleen. Isoform 1: Expressed in brain, spinal cord, muscle, heart, spleen and liver.
Function Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.
Protein Name Glycine--Trna Ligase
Diadenosine Tetraphosphate Synthetase
Ap4a Synthetase
Glycyl-Trna Synthetase
Glyrs
Glycyl-Trna Synthetase 1
Database Links Reactome: R-HSA-379716
Reactome: R-HSA-379726
Cellular Localisation Cytoplasm
Cell Projection
Axon
Secreted
Extracellular Exosome
In Transfected Cos7 Cells
Not Detected In Mitochondria
Nor In Golgi Apparatus
Secreted By Motor Neuron
Possibly Through The Exosome Pathway
Isoform 1: Mitochondrion
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Glycine--Trna Ligase antibody
Anti-Diadenosine Tetraphosphate Synthetase antibody
Anti-Ap4a Synthetase antibody
Anti-Glycyl-Trna Synthetase antibody
Anti-Glyrs antibody
Anti-Glycyl-Trna Synthetase 1 antibody
Anti-GARS1 antibody
Anti-GARS antibody

Information sourced from Uniprot.org

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