• Western blot detection of GAPDH (human specific) in Jurkat, COS7, K562 and Hela cell lysates using GAPDH (human specific) mouse mAb (1:3000 diluted).Predicted band size:37KDa.Observed band size:37KDa.
  • Western blot detection of GAPDH (human specific) stability in K562 cell lysate (A:Stored at room temperature for 72 hours;B:Stored at-20℃) using GAPDH (human specific) mouse mAb (1:10000 diluted).Predicted band size:37KDa.Observed band size:37KDa.
  • Western blot detection of GAPDH (human specific) in SW480, A431, K562, Jurkat and Hela cell lysates using GAPDH (human specific) mouse mAb (1:5000 diluted). Predicted band size:37KDa.Observed band size:37KDa.

Anti-GAPDH antibody [3C3-A2-E10] (STJ99065)

SKU:
STJ99065

Current Stock:
Host: Mouse
Applications: WB
Reactivity: Human/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Glyceraldehyde-3-phosphate dehydrogenase is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: 3C3-A2-E10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GAPDH
Gene ID: 2597
Uniprot ID: G3P_HUMAN
Specificity: This antibody detects endogenous levels of human GAPDH and does not cross-react with related proteins.
Immunogen: Purified recombinant human GAPDH protein fragments expressed in E.coli.
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Protein Name Glyceraldehyde-3-Phosphate Dehydrogenase
Gapdh
Peptidyl-Cysteine S-Nitrosylase Gapdh
Database Links Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Perinuclear Region
Membrane
Cytoskeleton
Translocates To The Nucleus Following S-Nitrosylation And Interaction With Siah1
Which Contains A Nuclear Localization Signal
Postnuclear And Perinuclear Regions
Alternative Antibody Names Anti-Glyceraldehyde-3-Phosphate Dehydrogenase antibody
Anti-Gapdh antibody
Anti-Peptidyl-Cysteine S-Nitrosylase Gapdh antibody
Anti-GAPDH antibody
Anti-GAPD antibody
Anti-CDABP0047 antibody
Anti-OK antibody
Anti-SW-cl.12 antibody

Information sourced from Uniprot.org

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