• Western blot analysis of extracts of various cell lines, using FXR1 rabbit monoclonal antibody (STJ11102355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using FXR1 Rabbit monoclonal antibody (STJ11102355) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using FXR1 rabbit monoclonal antibody (STJ11102355) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using FXR1 Rabbit monoclonal antibody (STJ11102355) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using FXR1 rabbit monoclonal antibody (STJ11102355) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using FXR1 Rabbit monoclonal antibody (STJ11102355) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using FXR1 rabbit monoclonal antibody (STJ11102355) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using FXR1 Rabbit monoclonal antibody (STJ11102355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FXR1 antibody (1-100) [S5MR] (STJ11102355)

SKU:
STJ11102355

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FXR1 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FXR1
Gene ID: 8087
Uniprot ID: FXR1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FXR1 (P51114).
Immunogen Sequence: MAELTVEVRGSNGAFYKGFI KDVHEDSLTVVFENNWQPER QVPFNEVRLPPPPDIKKEIS EGDEVEVYSRANDQEPCGWW LAKVRMMKGEFYVIEYAACD
Tissue Specificity Expressed in all tissues examined including heart, brain, kidney and testis. In brain, present at high level in neurons and especially in the Purkinje cells at the interface between the granular layer and the molecular layer (at protein level).
Post Translational Modifications Phosphorylation at Ser-420 by PAK1 promotes its relocalization to stress granules and activity. Phosphorylated by MAPK1/ERK2, promoting subsequent phosphorylation by GSK3B. Phosphorylated by GSK3B, promoting ubiquitination and degradation by the proteasome. Ubiquitinated by the SCF(FBXO4) complex, leading to its degradation by the proteasome: ubiquitination by the SCF(FBXO4) complex takes place following phosphorylation by GSK3B. Ubiquitinated and degraded in a GSK3B-dependent manner in during both scaling and sleep deprivation. Ubiquitinated via 'Lys-63'-linked ubiquitin, leading to its degradation: interaction with CEP63 inhibits 'Lys-63'-linked ubiquitination.
Function mRNA-binding protein that acts as a regulator of mRNAs translation and/or stability, and which is required for various processes, such as neurogenesis, muscle development and spermatogenesis. Specifically binds to AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Promotes formation of some phase-separated membraneless compartment by undergoing liquid-liquid phase separation upon binding to AREs-containing mRNAs, leading to assemble mRNAs into cytoplasmic ribonucleoprotein granules that concentrate mRNAs with associated regulatory factors. Required to activate translation of stored mRNAs during late spermatogenesis: acts by undergoing liquid-liquid phase separation to assemble target mRNAs into cytoplasmic ribonucleoprotein granules that recruit translation initiation factor EIF4G3 to activate translation of stored mRNAs in late spermatids. Promotes translation of MYC transcripts by recruiting the eIF4F complex to the translation start site. Acts as a negative regulator of inflammation in response to IL19 by promoting destabilization of pro-inflammatory transcripts. Also acts as an inhibitor of inflammation by binding to TNF mRNA, decreasing TNF protein production. Acts as a negative regulator of AMPA receptor GRIA2/GluA2 synthesis during long-lasting synaptic potentiation of hippocampal neurons by binding to GRIA2/GluA2 mRNA, thereby inhibiting its translation. Regulates proliferation of adult neural stem cells by binding to CDKN1A mRNA and promoting its expression. Acts as a regulator of sleep and synaptic homeostasis by regulating translation of transcripts in neurons. Required for embryonic and postnatal development of muscle tissue by undergoing liquid-liquid phase separation to assemble target mRNAs into cytoplasmic ribonucleoprotein granules. Involved in the nuclear pore complex localization to the nuclear envelope by preventing cytoplasmic aggregation of nucleoporins: acts by preventing ectopic phase separation of nucleoporins in the cytoplasm via a microtubule-dependent mechanism.
Protein Name Rna-Binding Protein Fxr1
Fmr1 Autosomal Homolog 1
Hfxr1p
Database Links Reactome: R-HSA-6802952
Cellular Localisation Cytoplasm
Cytoplasmic Ribonucleoprotein Granule
Stress Granule
Cell Projection
Dendrite
Dendritic Spine
Axon
Nucleus Envelope
Postsynapse
Specifically Localizes To Cytoplasmic Ribonucleoprotein Membraneless Compartments
Localizes To Stress Granules Following Phosphorylation At Ser-420 By Pak1
Adjacent To Z-Lines In Muscles
Alternative Antibody Names Anti-Rna-Binding Protein Fxr1 antibody
Anti-Fmr1 Autosomal Homolog 1 antibody
Anti-Hfxr1p antibody
Anti-FXR1 antibody

Information sourced from Uniprot.org

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