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Western blot analysis of extracts of various cell lines, using FUBP1 antibody (STJ11103646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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Confocal imaging of HeLa cells using FUBP1 Rabbit monoclonal antibody (STJ11103646, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
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Immunohistochemistry of paraffin-embedded rat brain using FUBP1 rabbit monoclonal antibody (STJ11103646) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
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Confocal imaging of paraffin-embedded Human breast cancer tissue using FUBP1 Rabbit monoclonal antibody (STJ11103646, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
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Immunohistochemistry of paraffin-embedded human colon using FUBP1 rabbit monoclonal antibody (STJ11103646) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
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Confocal imaging of U-2 OS cells using FUBP1 Rabbit monoclonal antibody (STJ11103646, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
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Immunohistochemistry of paraffin-embedded mouse testis using FUBP1 rabbit monoclonal antibody (STJ11103646) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
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Immunohistochemistry analysis of FUBP1 in paraffin-embedded mouse intestin tissue using FUBP1 Rabbit monoclonal antibody (STJ11103646) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunofluorescence analysis of U-2 OS cells using FUBP1 rabbit monoclonal antibody (STJ11103646) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunohistochemistry analysis of FUBP1 in paraffin-embedded mouse spleen tissue using FUBP1 Rabbit monoclonal antibody (STJ11103646) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of FUBP1 in paraffin-embedded rat lung tissue using FUBP1 Rabbit monoclonal antibody (STJ11103646) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of FUBP1 in paraffin-embedded rat intestine tissue using FUBP1 Rabbit monoclonal antibody (STJ11103646) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of FUBP1 in paraffin-embedded human esophagus tissue using FUBP1 Rabbit monoclonal antibody (STJ11103646) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of FUBP1 in paraffin-embedded mouse lung tissue using FUBP1 Rabbit monoclonal antibody (STJ11103646) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of FUBP1 in paraffin-embedded rat liver tissue using FUBP1 Rabbit monoclonal antibody (STJ11103646) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of FUBP1 in paraffin-embedded human breast cancer tissue using FUBP1 Rabbit monoclonal antibody (STJ11103646) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of FUBP1 in paraffin-embedded human thyroid tissue using FUBP1 Rabbit monoclonal antibody (STJ11103646) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Western blot analysis of various lysates using FUBP1 Rabbit monoclonal antibody (STJ11103646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
