• Western blot analysis of extracts of HepG2 cells, using FUBP1 antibody (STJ114417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FUBP1 antibody (1-280) (STJ114417)

SKU:
STJ114417

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FUBP1 (1-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FUBP1
Gene ID: 8880
Uniprot ID: FUBP1_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human FUBP1 (NP_003893.2).
Immunogen Sequence: MADYSTVPPPSSGSAGGGGG GGGGGGVNDAFKDALQRARQ IAAKIGGDAGTSLNSNDYGY GGQKRPLEDGDQPDAKKVAP QNDSFGTQLPPMHQQQSRSV MTEEYKVPDGMVGFIIGRGG EQISRIQQESGCKIQIAPDS GGLPERSCMLTGTPESVQSA KRLLDQIVEKGRPAPGFHHG DGPGNAVQEIMIPASKAGLV IGKGGETIKQLQERAGVKMV MIQDGPQNTGADKPLRITG
Post Translational Modifications Ubiquitinated. This targets the protein for proteasome-mediated degradation.
Function Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
Protein Name Far Upstream Element-Binding Protein 1
Fbp
Fuse-Binding Protein 1
Dna Helicase V
Hdh V
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Far Upstream Element-Binding Protein 1 antibody
Anti-Fbp antibody
Anti-Fuse-Binding Protein 1 antibody
Anti-Dna Helicase V antibody
Anti-Hdh V antibody
Anti-FUBP1 antibody

Information sourced from Uniprot.org

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