• Western blot analysis of extracts of various cell lines, using Fascin/FSCN1 rabbit monoclonal antibody (STJ11102495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using Fascin/FSCN1 Rabbit monoclonal antibody (STJ11102495) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat spleen using Fascin/FSCN1 rabbit monoclonal antibody (STJ11102495) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded Human Hodgkin lymphoma using Fascin/FSCN1 Rabbit monoclonal antibody (STJ11102495) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using Fascin/FSCN1 rabbit monoclonal antibody (STJ11102495) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded Human anaplastic large cell lymphoma using Fascin/FSCN1 Rabbit monoclonal antibody (STJ11102495) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using Fascin/FSCN1 rabbit monoclonal antibody (STJ11102495) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Fascin/FSCN1 Rabbit monoclonal antibody (STJ11102495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FSCN1 antibody (249-362) [S5MR] (STJ11102495)

SKU:
STJ11102495

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Fascin (249-362) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FSCN1
Gene ID: 6624
Uniprot ID: FSCN1_HUMAN
Immunogen Region: 249-362
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 249-362 of human Fascin/FSCN1 (Q16658).
Immunogen Sequence: GKDELFALEQSCAQVVLQAA NERNVSTRQGMDLSANQDEE TDQETFQLEIDRDTKKCAFR THTGKYWTLTATGGVQSTAS SKNASCYFDIEWRDRRITLR ASNGKFVTSKKNGQ
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylation at Ser-39 inhibits actin-binding. Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF.
Function Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF.
Protein Name Fascin
55 Kda Actin-Bundling Protein
Singed-Like Protein
P55
Database Links Reactome: R-HSA-6785807
Cellular Localisation Cytoplasm
Cytosol
Cell Cortex
Cytoskeleton
Stress Fiber
Cell Projection
Filopodium
Invadopodium
Microvillus
Cell Junction
Colocalized With Rufy3 And F-Actin At Filipodia Of The Axonal Growth Cone
Colocalized With Dbn1 And F-Actin At The Transitional Domain Of The Axonal Growth Cone
Alternative Antibody Names Anti-Fascin antibody
Anti-55 Kda Actin-Bundling Protein antibody
Anti-Singed-Like Protein antibody
Anti-P55 antibody
Anti-FSCN1 antibody
Anti-FAN1 antibody
Anti-HSN antibody
Anti-SNL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance