• Western blot analysis of extracts of various cell lines, using FRK antibody (STJ29694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FRK antibody (1-220) (STJ29694)

SKU:
STJ29694

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FRK (1-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FRK
Gene ID: 2444
Uniprot ID: FRK_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human FRK (NP_002022.1).
Immunogen Sequence: MSNICQRLWEYLEPYLPCLS TEADKSTVIENPGALCSPQS QRHGHYFVALFDYQARTAED LSFRAGDKLQVLDTLHEGWW FARHLEKRRDGSSQQLQGYI PSNYVAEDRSLQAEPWFFGA IGRSDAEKQLLYSENKTGSF LIRESESQKGEFSLSVLDGA VVKHYRIKRLDEGGFFLTRR RIFSTLNEFVSHYTKTSDGL CVKLGKPCLKIQVPAPFDLS
Tissue Specificity Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon.
Function Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
Protein Name Tyrosine-Protein Kinase Frk
Fyn-Related Kinase
Nuclear Tyrosine Protein Kinase Rak
Protein-Tyrosine Kinase 5
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8948751
Cellular Localisation Cytoplasm
Nucleus
Predominantly Found In The Nucleus
With A Small Fraction Found In The Cell Periphery
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Frk antibody
Anti-Fyn-Related Kinase antibody
Anti-Nuclear Tyrosine Protein Kinase Rak antibody
Anti-Protein-Tyrosine Kinase 5 antibody
Anti-FRK antibody
Anti-PTK5 antibody
Anti-RAK antibody

Information sourced from Uniprot.org

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