| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FRK (1-220) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FRK |
| Gene ID: |
2444 |
| Uniprot ID: |
FRK_HUMAN |
| Immunogen Region: |
1-220 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human FRK (NP_002022.1). |
| Immunogen Sequence: |
MSNICQRLWEYLEPYLPCLS TEADKSTVIENPGALCSPQS QRHGHYFVALFDYQARTAED LSFRAGDKLQVLDTLHEGWW FARHLEKRRDGSSQQLQGYI PSNYVAEDRSLQAEPWFFGA IGRSDAEKQLLYSENKTGSF LIRESESQKGEFSLSVLDGA VVKHYRIKRLDEGGFFLTRR RIFSTLNEFVSHYTKTSDGL CVKLGKPCLKIQVPAPFDLS |
| Tissue Specificity | Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon. |
| Function | Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor. |
| Protein Name | Tyrosine-Protein Kinase FrkFyn-Related KinaseNuclear Tyrosine Protein Kinase RakProtein-Tyrosine Kinase 5 |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-8948751 |
| Cellular Localisation | CytoplasmNucleusPredominantly Found In The NucleusWith A Small Fraction Found In The Cell Periphery |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Frk antibodyAnti-Fyn-Related Kinase antibodyAnti-Nuclear Tyrosine Protein Kinase Rak antibodyAnti-Protein-Tyrosine Kinase 5 antibodyAnti-FRK antibodyAnti-PTK5 antibodyAnti-RAK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Emerin/Emerin/EMD knockout (KO) 293T cells, using [KO Validated] Emerin/EMD Rabbit polyclonal antibody (STJ119989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98774/382822/STJ119989_1__22320.1713151321.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Emerin/Emerin/EMD knockout (KO) 293T cells, using [KO Validated] Emerin/EMD Rabbit polyclonal antibody (STJ119989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")