• Western blot analysis of extracts of various cell lines, using FOSL2 antibody (STJ23690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human esophagus using FOSL2 antibody (STJ23690) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of U-2 OS cells using FOSL2 antibody (STJ23690) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-FOSL2 antibody (187-326) (STJ23690)

SKU:
STJ23690

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FOSL2 (187-326) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FOSL2
Gene ID: 2355
Uniprot ID: FOSL2_HUMAN
Immunogen Region: 187-326
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 187-326 of human FOSL2 (NP_005244.1).
Immunogen Sequence: HGPVCKISPEERRSPPAPGL QPMRSGGGSVGAVVVKQEPL EEDSPSSSSAGLDKAQRSVI KPISIAGGFYGEEPLHTPIV VTSTPAVTPGTSNLVFTYPS VLEQESPASPSESCSKAHRR SSSSGDQSSDSLNSPTLLAL
Function Controls osteoclast survival and size. As a dimer with JUN, activates LIF transcription. Activates CEBPB transcription in PGE2-activated osteoblasts.
Protein Name Fos-Related Antigen 2
Fra-2
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Fos-Related Antigen 2 antibody
Anti-Fra-2 antibody
Anti-FOSL2 antibody
Anti-FRA2 antibody

Information sourced from Uniprot.org

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