• Western blot analysis of extracts of A-549 cells, using EDN3 antibody (STJ26165) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 20s.

Anti-EDN3 antibody (17-187) (STJ26165)

SKU:
STJ26165

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EDN3 (17-187) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EDN3
Gene ID: 1908
Uniprot ID: EDN3_HUMAN
Immunogen Region: 17-187
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 17-187 of human EDN3 (NP_996917.1).
Immunogen Sequence: AGFVPCSQSGDAGRRGVSQA PTAARSEGDCEETVAGPGEE TVAGPGEGTVAPTALQGPSP GSPGQEQAAEGAPEHHRSRR CTCFTYKDKECVYYCHLDII WINTPEQTVPYGLSNYRGSF RGKRSAGPLPGNLQLSHRPH LRCACVGRYDKACLHFCTQT LDVSSNSRTAE
Tissue Specificity Expressed in trophoblasts and placental stem villi vessels, but not in cultured placental smooth muscle cells.
Function Endothelins are endothelium-derived vasoconstrictor peptides.
Protein Name Endothelin-3
Et-3
Preproendothelin-3
Ppet3
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-416476
Cellular Localisation Secreted
Alternative Antibody Names Anti-Endothelin-3 antibody
Anti-Et-3 antibody
Anti-Preproendothelin-3 antibody
Anti-Ppet3 antibody
Anti-EDN3 antibody

Information sourced from Uniprot.org

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