| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FHIT (25-147) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FHIT |
| Gene ID: |
2272 |
| Uniprot ID: |
FHIT_HUMAN |
| Immunogen Region: |
25-147 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human FHIT (NP_001159715.1). |
| Immunogen Sequence: |
LVNRKPVVPGHVLVCPLRPV ERFHDLRPDEVADLFQTTQR VGTVVEKHFHGTSLTFSMQD GPEAGQTVKHVHVHVLPRKA GDFHRNDSIYEELQKHDKED FPASWRSEEEMAAEAAALRV YFQ |
| Tissue Specificity | Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. |
| Post Translational Modifications | Phosphorylation at Tyr-114 by SRC is required for induction of apoptosis. |
| Function | Possesses dinucleoside triphosphate hydrolase activity. Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Exhibits adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate to yield AMP and sulfate. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Exhibits adenylylsulfate-ammonia adenylyltransferase, catalyzing the ammonolysis of adenosine 5'-phosphosulfate resulting in the formation of adenosine 5'-phosphoramidate. Also catalyzes the ammonolysis of adenosine 5-phosphorofluoridate and diadenosine triphosphate. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor. |
| Protein Name | Bis(5'-Adenosyl-TriphosphataseAp3a HydrolaseAp3aaseAdenosine 5'-Monophosphoramidase FhitAdenylylsulfataseAdenylylsulfate-Ammonia AdenylyltransferaseDiadenosine 5' -5'''-P1 -P3-Triphosphate HydrolaseDinucleosidetriphosphataseFragile Histidine Triad Protein |
| Cellular Localisation | CytoplasmMitochondrionNucleus |
| Alternative Antibody Names | Anti-Bis(5'-Adenosyl-Triphosphatase antibodyAnti-Ap3a Hydrolase antibodyAnti-Ap3aase antibodyAnti-Adenosine 5'-Monophosphoramidase Fhit antibodyAnti-Adenylylsulfatase antibodyAnti-Adenylylsulfate-Ammonia Adenylyltransferase antibodyAnti-Diadenosine 5' -5'''-P1 -P3-Triphosphate Hydrolase antibodyAnti-Dinucleosidetriphosphatase antibodyAnti-Fragile Histidine Triad Protein antibodyAnti-FHIT antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
