• Western blot analysis of lysates from Mouse brain, using FGD5 Rabbit polyclonal antibody (STJ117406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-FGD5 antibody (1000-1200) (STJ117406)

SKU:
STJ117406

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FGD5 (1000-1200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FGD5
Gene ID: 152273
Uniprot ID: FGD5_HUMAN
Immunogen Region: 1000-1200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1200 of human FGD5 (NP_689749.3).
Immunogen Sequence: YLGLLSENCLHSPRLAAAVR EFEQSVQGGSQTAKHRLLRV VQRLFQYQVLLTDYLNNLCP DSAEYDNTQGALSLISKVTD RANDSMEQGENLQKLVHIEH SVRGQGDLLQPGREFLKEGT LMKVTGKNRRPRHLFLMNDV LLYTYPQKDGKYRLKNTLAV ANMKVSRPVMEKVPYALKIE TSESCLMLSASSCAERDEWY G
Tissue Specificity Expressed in endothelial cells (at protein level).
Function Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Mediates VEGF-induced CDC42 activation. May regulate proangiogenic action of VEGF in vascular endothelial cells, including network formation, directional movement and proliferation. May play a role in regulating the actin cytoskeleton and cell shape.
Protein Name Fyve - Rhogef And Ph Domain-Containing Protein 5
Zinc Finger Fyve Domain-Containing Protein 23
Database Links Reactome: R-HSA-9013149
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Ruffle Membrane
Endoplasmic Reticulum
Golgi Apparatus
Early Endosome
In Peripheral Membrane Ruffles
Colocolizes With F-Actin
In Confluent Huvecs
Detected At Cell-Cell-Contact Sites Where It Colocalizes With Vascular Endothelial Cadherin/Cdh5
Alternative Antibody Names Anti-Fyve - Rhogef And Ph Domain-Containing Protein 5 antibody
Anti-Zinc Finger Fyve Domain-Containing Protein 23 antibody
Anti-FGD5 antibody
Anti-ZFYVE23 antibody

Information sourced from Uniprot.org

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