| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FBXO5 (1-250) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FBXO5 |
| Gene ID: |
26271 |
| Uniprot ID: |
FBX5_HUMAN |
| Immunogen Region: |
1-250 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human FBXO5 (NP_036309.1). |
| Immunogen Sequence: |
MSRRPCSCALRPPRCSCSAS PSAVTAAGRPRPSDSCKEES STLSVKMKCDFNCNHVHSGL KLVKPDDIGRLVSYTPAYLE GSCKDCIKDYERLSCIGSPI VSPRIVQLETESKRLHNKEN QHVQQTLNSTNEIEALETSR LYEDSGYSSFSLQSGLSEHE EGSLLEENFGDSLQSCLLQI QSPDQYPNKNLLPVLHFEKV VCSTLKKNAKRNPKVDREML KEIIARGNFRLQNIIGRKM |
| Post Translational Modifications | Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression. Phosphorylated by RPS6KA2.increases and stabilizes interaction with CDC20. Ubiquitinated by the SCF(BTRC) complex following phosphorylation by PLK1. Undergoes both 'Lys-11' and 'Lys-48'-linked polyubiquitination by APC-FZR1 complex leading to degradation by proteasome during G1 phase. Degraded through the SCF(BTRC) complex.degradation occurs during oocyte maturation, between germinal vesicle breakdown (GVBD) and meiosis I, and is required for the meiosis I-meiosis II transition. |
| Function | Regulator of APC activity during mitotic and meiotic cell cycle. During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex. During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase. Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment. As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box coreceptor, formed by FZR1 and ANAPC10.by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities. Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence. During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization. Controls entry into the first meiotic division through inactivation of APC-FZR1 complex. Promotes migration and osteogenic differentiation of mesenchymal stem cells. |
| Protein Name | F-Box Only Protein 5Early Mitotic Inhibitor 1 |
| Database Links | Reactome: R-HSA-174113Reactome: R-HSA-176408Reactome: R-HSA-176417Reactome: R-HSA-68881Reactome: R-HSA-69205 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindleIn InterphaseLocalizes In A Punctate Manner In The Nucleus And Cytoplasm With Some Perinuclear ConcentrationIn Mitotic CellsLocalizes Throughout The CellParticularly At The Spindle |
| Alternative Antibody Names | Anti-F-Box Only Protein 5 antibodyAnti-Early Mitotic Inhibitor 1 antibodyAnti-FBXO5 antibodyAnti-EMI1 antibodyAnti-FBX5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
