| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:4000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FBXO4 |
| Gene ID: | 26272 |
| Uniprot ID: | FBX4_HUMAN |
| Immunogen Region: | 138-387 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 138-387 of human FBXO4 (NP_036308.1). |
| Immunogen Sequence: | DYMAVYRMCCPYTRRASKSS RPMYGAVTSFLHSLIIQNEP RFAMFGPGLEELNTSLVLSL MSSEELCPTAGLPQRQIDGI GSGVNFQLNNQHKFNILILY STTRKERDRAREEHTSAVNK MFSRHNEGDDQQGSRYSVIP QIQKVCEVVDGFIYVANAEA HKRHEWQDEFSHIMAMTDPA FGSSGRPLLVLSCISQGDVK RMPCFYLAHELHLNLLNHPW LVQDTEAETLTGFLNGIEW |
| Post Translational Modifications | Phosphorylation at Ser-12 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase. Phosphorylation at Ser-12 promotes homodimerization and is necessary for optimal ubiquitin ligase activity towards CCND1. |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1. Promotes ubiquitination of FXR1 following phosphorylation of FXR1 by GSK3B, leading to FXR1 degradation by the proteasome. |
| Protein Name | F-Box Only Protein 4 |
| Database Links | Reactome: R-HSA-390471Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-F-Box Only Protein 4 antibodyAnti-FBXO4 antibodyAnti-FBX4 antibody |
Information sourced from Uniprot.org