• Confocal imaging of U-2OS cells using Fibrillarin/U3 RNP Rabbit monoclonal antibody (STJ11101258, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Western blot analysis of various lysates using Fibrillarin/U3 RNP Rabbit monoclonal antibody (STJ11101258) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates using Fibrillarin/U3 RNP Rabbit monoclonal antibody (STJ11101258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-FBL antibody (222-321) [S8MR] (STJ11101258)

SKU:
STJ11101258

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Fibrillarin (222-321) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FBL
Gene ID: 2091
Uniprot ID: FBRL_HUMAN
Immunogen Region: 222-321
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 222-321 of human Fibrillarin/U3 RNP (P22087).
Immunogen Sequence: KYRMLIAMVDVIFADVAQPD QTRIVALNAHTFLRNGGHFV ISIKANCIDSTASAEAVFAS EVKKMQQENMKPQEQLTLEP YERDHAVVVGVYRPPPKVKN
Post Translational Modifications Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36. By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA). Acetylated by CREBBP/CBP, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me), without affecting rRNA methylation. Deacetylation by SIRT7 restores methylation of 'Gln-105' of histone H2A (H2AQ104me).
Function S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes. U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name Rrna 2'-O-Methyltransferase Fibrillarin
34 Kda Nucleolar Scleroderma Antigen
Histone-Glutamine Methyltransferase
U6 Snrna 2'-O-Methyltransferase Fibrillarin
Database Links Reactome: R-HSA-6790901
Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Fibrillar Region Of The Nucleolus
Alternative Antibody Names Anti-Rrna 2'-O-Methyltransferase Fibrillarin antibody
Anti-34 Kda Nucleolar Scleroderma Antigen antibody
Anti-Histone-Glutamine Methyltransferase antibody
Anti-U6 Snrna 2'-O-Methyltransferase Fibrillarin antibody
Anti-FBL antibody
Anti-FIB1 antibody
Anti-FLRN antibody

Information sourced from Uniprot.org

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