| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ERLIN2 |
| Gene ID: | 11160 |
| Uniprot ID: | ERLN2_HUMAN |
| Immunogen Region: | 25-152 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-152 of human ERLIN2 (NP_001003790.1). |
| Immunogen Sequence: | KIEEGHIGVYYRGGALLTST SGPGFHLMLPFITSYKSVQT TLQTDEVKNVPCGTSGGVMI YFDRIEVVNFLVPNAVYDIV KNYTADYDKALIFNKIHHEL NQFCSVHTLQEVYIELFGLE NDFSQESS |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Deubiquitinated by USP25.leading to stabilization. |
| Function | Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1. Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex. Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex. |
| Protein Name | Erlin-2 Endoplasmic Reticulum Lipid Raft-Associated Protein 2 Stomatin-Prohibitin-Flotillin-Hflc/K Domain-Containing Protein 2 Spfh Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-382556 Reactome: R-HSA-5655302 Reactome: R-HSA-5678895 Reactome: R-HSA-8853336 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Type Ii Membrane Protein Associated With Lipid Raft-Like Domains Of The Endoplasmic Reticulum Membrane |
| Alternative Antibody Names | Anti-Erlin-2 antibody Anti-Endoplasmic Reticulum Lipid Raft-Associated Protein 2 antibody Anti-Stomatin-Prohibitin-Flotillin-Hflc/K Domain-Containing Protein 2 antibody Anti-Spfh Domain-Containing Protein 2 antibody Anti-ERLIN2 antibody Anti-C8orf2 antibody Anti-SPFH2 antibody Anti-UNQ2441 antibody Anti-PRO5003 antibody Anti-PRO9924 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and CNPasease knockout (KO) HeLa cells, using [KO Validated] CNPase Rabbit polyclonal antibody (STJ119982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98767/382818/STJ119982_1__79726.1713151315.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CNPasease knockout (KO) HeLa cells, using [KO Validated] CNPase Rabbit polyclonal antibody (STJ119982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")