• Western blot analysis of extracts of various cell lines, using ERCC6L antibody (STJ27892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ERCC6L antibody (300-400) (STJ27892)

SKU:
STJ27892

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ERCC6L (300-400) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERCC6L
Gene ID: 54821
Uniprot ID: ERC6L_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human ERCC6L (NP_060139.2).
Immunogen Sequence: KDATPGEKALGFKISENLMA IIKPYFLRRTKEDVQKKKSS NPEARLNEKNPDVDAICEMP SLSRKNDLIIWIRLVPLQEE IYRKFVSLDHIKELLMETRS P
Post Translational Modifications Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.
Function DNA helicase that acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. Functions as ATP-dependent DNA translocase. Can promote Holliday junction branch migration (in vitro).
Protein Name Dna Excision Repair Protein Ercc-6-Like
Atp-Dependent Helicase Ercc6-Like
Plk1-Interacting Checkpoint Helicase
Tumor Antigen Bj-Hcc-15
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Chromosome
Centromere
Kinetochore
Localizes To Kinetochores
Inner Centromeres And Thin Threads Connecting Separating Chromosomes Even During Anaphase
In Prometaphase Cells
It Mostly Concentrates In Between Kinetochores
In Metaphase
It Localizes To Numerous Thin Threads That Stretch Between Sister Kinetochores Of The Aligned Chromosomes And Are Composed Of Catenated Centromeric Dna
Evolution From Inner Centromeres To Thin Threads Takes Place In Response To Tension
Resolution Of Thin Threads Requires Topoisomerase 2-Alpha (Top2a) After Anaphase Onset
Alternative Antibody Names Anti-Dna Excision Repair Protein Ercc-6-Like antibody
Anti-Atp-Dependent Helicase Ercc6-Like antibody
Anti-Plk1-Interacting Checkpoint Helicase antibody
Anti-Tumor Antigen Bj-Hcc-15 antibody
Anti-ERCC6L antibody
Anti-PICH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance